Several worlds in our solar system are thought to hold oceans of liquid water beneath their frozen surfaces. These subsurface ice and ocean environments are promising targets in the search for life beyond Earth, but they also present significant new technical challenges to planetary exploration. With a focus on Jupiter's moon Europa, here we (1) identify major benefits and challenges to subsurface ocean world science, (2) provide a multidisciplinary survey of relevant sample handling and life detection technologies, and (3) integrate those perspectives into the Subsurface Science and Search for Life in Ocean Worlds (SSSLOW) concept payload. We discuss scientific goals across three complementary categories: (1) search for life, (2) assess habitability, and (3) investigate geological processes. Major mission challenges considered include submerged operation in high-pressure environments, the need to sample fluids with a range of possible chemical conditions, and detection of biosignatures at low concentrations. The SSSLOW addresses these issues by tightly integrated instrumentation and sample handling systems to enable sequential, complementary measurements while prioritizing preservation of sample context. In this work, we leverage techniques and technologies across several fields to demonstrate a path toward future subsurface exploration and life detection in ice and ocean worlds.

Ice streams that flow into Ross Ice Shelf are underlain by water-saturated sediments, a dynamic hydrological system, and subglacial lakes that intermittently discharge water downstream across grounding zones of West Antarctic Ice Sheet (WAIS). A 2.06 m composite sediment profile was recently recovered from Mercer Subglacial Lake, a 15 m deep water cavity beneath a 1087 m thick portion of the Mercer Ice Stream. We examined microbial abundances, used 16S rRNA gene amplicon sequencing to assess community structures, and characterized extracellular polymeric substances (EPS) associated with distinct lithologic units in the sediments. Bacterial and archaeal communities in the surficial sediments are more abundant and diverse, with significantly different compositions from those found deeper in the sediment column. The most abundant taxa are related to chemolithoautotrophs capable of oxidizing reduced nitrogen, sulfur, and iron compounds with oxygen, nitrate, or iron. Concentrations of dissolved methane and total organic carbon together with water content in the sediments are the strongest predictors of taxon and community composition. δ¹³C values for EPS (–25 to –30%) are consistent with the primary source of carbon for biosynthesis originating from legacy marine organic matter. Comparison of communities to those in lake sediments under an adjacent ice stream (Whillans Subglacial Lake) and near its grounding zone provide seminal evidence for a subglacial metacommunity that is biogeochemically and evolutionarily linked through ice sheet dynamics and the transport of microbes, water, and sediments beneath WAIS.

The Subglacial Antarctic Lakes Scientific Access (SALSA) Project accessed Mercer Subglacial Lake using environmentally clean hot-water drilling to examine interactions among ice, water, sediment, rock, microbes and carbon reservoirs within the lake water column and underlying sediments. A similar to 0.4 m diameter borehole was melted through 1087 m of ice and maintained over similar to 10 days, allowing observation of ice properties and collection of water and sediment with various tools. Over this period, SALSA collected: 60 L of lake water and 10 L of deep borehole water; microbes >0.2 μm in diameter from in situ filtration of similar to 100 L of lake water; 10 multicores 0.32–0.49 m long; 1.0 and 1.76 m long gravity cores; three conductivity-temperature-depth profiles of borehole and lake water; five discrete depth current meter measurements in the lake and images of ice, the lake water–ice interface and lake sediments. Temperature and conductivity data showed the hydrodynamic character of water mixing between the borehole and lake after entry. Models simulating melting of the similar to 6 m thick basal accreted ice layer imply that debris fall-out through the similar to 15 m water column to the lake sediments from borehole melting had little effect on the stratigraphy of surficial sediment cores.

Geodetic observations in the oceans are important for understanding plate tectonics, earthquake cycles and volcanic processes. One approach to seafloor geodesy is the use of seafloor pressure gauges to sense vertical changes in the elevation of the seafloor after correcting for variations in the weight of the overlying oceans and atmosphere. A challenge of using pressure gauges is the tendency for the sensors to drift. The A-0-A method is a new approach for correcting drift. A valve is used to periodically switch, for a short time, the measured pressure from the external ocean to the inside of the instrument housing at atmospheric pressure. The internal pressure reading is compared to an accurate barometer to measure the drift which is assumed to be the same at low and high pressures. We describe a 30-months test of the A-0-A method at 900 m depth on the MARS cabled observatory in Monterey Bay using an instrument that includes two A-0-A calibrated pressure gauges and a three-component accelerometer. Prior to the calibrations, the two pressure sensors drift by 6 and 2 hPa, respectively. After the calibrations, the offsets of the corrected pressure sensors are consistent with each other to within 0.2 hPa. The drift corrected detided external pressure measurements show a 0.5 hPa/yr trend of increasing pressures during the experiment. The measurements are corrected for instrument subsidence based on the changes in tilt measured by the accelerometer, but the trend may include a component of subsidence that did not affect tilt. However, the observed trend of increasing pressure, closely matches that calculated from satellite altimetry and repeat conductivity, temperature and depth casts at a nearby location, and increasing pressures are consistent with the trend expected for the El Niño Southern Oscillation. We infer that the A-0-A drift corrections are accurate to better than one part in 10⁵ per year. Additional long-term tests and comparisons with oceanographic observations and other methods for drift correction will be required to understand if the accuracy the A-0-A drift corrections matches the observed one part in 10⁶ per year consistency between the two pressure sensors.

Understanding ice sheet evolution through the geologic past can help constrain ice sheet models that predict future ice dynamics. Existing geological records of grounding line retreat in the Ross Sea, Antarctica, have been confined to ice‐free and terrestrial archives, which reflect dynamics from periods of more extensive ice cover. Therefore, our perspective of grounding line retreat since the Last Glacial Maximum remains incomplete. Sediments beneath Ross Ice Shelf and grounded ice offer complementary insight into the southernmost extent of grounding line retreat, yielding a more complete view of ice dynamics during deglaciation. Here we thermochemically separate the youngest organic carbon to estimate ages from sediments extracted near the Whillans Ice Stream grounding line to provide direct evidence of mid‐Holocene (7.5–4.8 kyr B.P.) grounding line retreat in that region. Our study demonstrates the utility of accurately dated, grounding‐line‐proximal sediment deposits for reconstructing past interactions between marine and subglacial environments.